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. 2015 Sep 11;5:14016. doi: 10.1038/srep14016

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Copyright © 2015, Macmillan Publishers Limited

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(A) Visualization of RdRp^1–296-HSP20 interaction in rice protoplasts by BiFC assay. Rice protoplasts were co-transfected with recombinant BiFC vectors containing the constructs indicated above the images. The results were observed 18 h after transfection. Scale bar, 5 μm. (B) Visualization of RdRp^1–296-HSP20 interaction in N. benthamiana epidermal cells by BiFC assay. N. benthamiana leaves were co-infiltrated with recombinant BiFC vectors containing the constructs indicated above the images. The results were observed 48 h after infiltration. Scale bar, 50 μm. The fluorescent and merged images are depicted in the upper and lower panels, respectively.