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. 2015 Sep 11;5:14016. doi: 10.1038/srep14016

Figure 8. In vitro pull-down analysis of interactions between the N-terminus of RSV RdRp and host HSP20.

Figure 8

In vitro translated c-Myc-RdRp1–296 or c-Myc-p53 fusion protein was incubated with equal amount of OsHSP20-GFP, NbHSP20-GFP or GFP, and accreted to GFP-Trap M beads. Beads were washed and analyzed by western blot assays using an anti-c-Myc antibody (upper) or an anti-GFP antibody (lower). The two right membranes show the inputs of in vitro translated proteins in pull-down assays. Black arrows indicate the corresponding bands of c-Myc-p53, c-Myc-RdRp1–296, OsHSP20-GFP, NbHSP20-GFP and GFP, respectively.