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. 2015 Sep 11;5:14016. doi: 10.1038/srep14016

Table 1. Primers used in plasmid construction.

Primers Sequences (5′-3′) Restriction sites (underlined)
R1-1F GGAATTCCATATGACGACACCACCTCTCG NdeI
R1-1R CGCGGATCCTTACAGGACCCAGGTAGATCTG BamHI
R1-2F GGAATTCCATATGAAACCAGGAGGAAGCAGA NdeI
R1-2R CCGGAATTCCTCGGTGGCAAGATCAGAT EcoRI
R1-3F CGCGGATCCCTAGCCATCTCATTGTCC BamHI
R1-3R CGCGGATCCTGTCGTACAGGCAATCCA BamHI
R1-4F GGAATTCCATATGACACCTTTGGGAGAGAAG NdeI
R1-4R CGCGGATCCGTTCTCCTTCATTAGCTG BamHI
R1-5F CCGGAATTCACTCACCTAGGTGGTAGG EcoRI
R1-5R CGCGGATCCTCAGAAATCGAACTTATGGTC BamHI
Os20F GGAATTCCATATGTCGCTGATCCGCCGCAG NdeI
Os20R CGCGGATCCCTAGCCGGTAACCTGGATG BamHI
Nb20F GGAATTCCATATGTCTCTTATCCCAAGC NdeI
Nb20R CCGGAATTCTTAACCAGATATCTCAATGGC EcoRI
B1-1F CGCGGATCCATGACGACACCACCTCTCG BamHI
B1-1R GCGAGCTCTTACAGGACCCAGGTAGATCTG SacI
BOs20F CGCGGATCCATGTCGCTGATCCGCCGCAG BamHI
BOs20R GCGAGCTCCTAGCCGGTAACCTGGATG SacI
BNb20F CGGGGTACCATGTCTCTTATCCCAAGC KpnI
BNb20R GCGAGCTCTTAACCAGATATCTCAATGGC SacI
G1-1R ACGCGTCGACCAGGACCCAGGTAGATCTG SalI
GOs20R ACGCGTCGACGCCGGTAACCTGGATGGAC SalI
GNb20R CGGGGTACCACCAGATATCTCAATGGC KpnI
P1-1F GGGGTACCATGGAGGAGCAGAAGCTGATC KpnI
P1-1R ATAAGAATGCGGCCGCTTACAGGACCCAGGTAGATCTG NotI
POs20F CGGAATTCATGTCGCTGATCCGCCGCAG EcoRI
POs20R CGGGGTACCTTACTTGTACAGCTCGTCCATG KpnI
PNb20F CGGAATTCATGTCTCTTATCCCAAGC EcoRI
PNb20R ATAAGAATGCGGCCGCTTACTTGTACAGCTCGTCCATG NotI
GFPF CGGAATTCATGGTGAGCAAGGGCGAGG EcoRI
P53F ACGCGTCGACATGGAGGAGCAGAAGCTGATC SalI
P53R ATAAGAATGCGGCCGCTCAGTCTGAGTCAGGCCC NotI