Table 1.
Estimated concentrations of noro- and sapovirus, and detected genotypes in sea snail samples
| Month | Sample name | NoV GI | NoV GII | SaV | Efficiency rate (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Real-time PCRa (Copies/g) | Conventional PCR | Genotype | Real-time PCRa(Copies/g) | Conventional PCR | Genotype | Real-time PCRa(Copies/g) | Conventional PCR | Genotype | |||
| May-2012 | nagarami A | 4.0 × 102 | − | − | 7.9 × 103 | − | − | + | + | GI.3 | 42.7 |
| nagarami B | − | − | − | 1.5 × 103 | − | − | − | + | GI.2 | 38.7 | |
| nagarami C | − | − | − | − | − | − | + | + | GI.3 | 58.9 | |
| nagarami D | − | + | GI.4 | 4.0 × 103 | + | GII.14 | 1.5 × 102 | − | − | 100 | |
| nagarami E | − | − | − | 3.3 × 103 | + | GII.4 | + | − | − | 100 | |
| Jun-2012 | nagarami F | − | − | − | − | + | GII.4 | + | − | − | 100 |
| nagarami G | − | − | − | − | − | − | − | − | − | 76.7 | |
| nagarami H | − | − | − | − | − | − | − | − | − | 51.5 | |
| nagarami I | − | − | − | − | − | − | − | − | − | 72.8 | |
| nagarami J | − | − | − | − | − | − | − | − | − | 74.9 | |
| Aug-2012 | nagarami K | − | − | − | − | − | − | − | − | − | 56.1 |
| nagarami L | − | − | − | − | − | − | − | − | − | 39.1 | |
| nagarami M | − | − | − | − | − | − | − | − | − | 61.7 | |
| nagarami N | − | − | − | − | − | − | − | − | − | 24.9 | |
| nagarami O | − | − | − | − | − | − | − | − | − | 38.8 | |
| Oct-2012 | nagarami P | + | − | − | − | − | − | − | − | − | 100 |
| nagarami Q | + | − | − | − | − | − | − | − | − | 78.7 | |
| nagarami R | − | − | − | − | − | − | − | − | − | 85.3 | |
| nagarami S | − | − | − | − | − | − | − | − | − | 63.0 | |
| nagarami T | − | − | − | 4.7 × 103 | − | − | 5.8 × 102 | − | − | 75.7 | |
| Dec-2012 | nagarami U | 4.5 × 103 | + | GI.4 | 6.9 × 104 | + | GII.4 | 2.8 × 102 | − | − | 100 |
| nagarami V | 2.7 × 102 | − | − | 2.2 × 104 | + | GII.4 | + | − | − | 100 | |
| nagarami W | − | + | GI.6 | 3.2 × 104 | + | GII.13 | − | + | GI.2 | 53.0 | |
| nagarami X | + | − | − | − | + | GII.4 | − | − | − | 50.3 | |
| nagarami Y | 1.7 × 103 | − | − | 5.6 × 104 | + | GII.12 | − | − | − | 91.5 | |
| nagarami Z | 4.7 × 103 | + | GI.6 | 7.6 × 104 | + | GII.4 | 3.7 × 102 | − | − | 39.1 | |
| nagarami AA | 2.8 × 103 | − | – | 1.4 × 105 | + | GII.4 | 1.3 × 103 | − | − | 49.7 | |
aIn real-time PCR, Numbers represent copy numbers that displayed amplification in two wells and more than the limit of quantification. + indicated less than the limit of quantification