Figure 4.

Bandpass limited imaging modes of the RIS for assessing subretinal injection efficiency and performing far red imaging. Enhanced green fluorescent protein expression assessed in vivo in the retina of a C1xC57BL/6 mouse that received a subretinal injection of AAV-EGFP virus (1.0 μL) at 14 days of age. The bright field (A) and fluorescence images (B) were taken 12 weeks post injection. Bandpass filters were used to excite the EGFP and capture its emission for fluorescence imaging. In vivo imaging of C1xBL6 mouse (∼2 months of age) retina using a far red bandpass filter (692 nm peak, 40 nm bandpass) (C). This allows examination of the posterior pole and vasculature during dark-adapted studies or during subretinal injections in light sensitive animal models (to prevent light damage).