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. 2015 Nov 11;56(12):7338–7344. doi: 10.1167/iovs.15-18038

Figure 6.

Figure 6

Three-dimensional quantification of sox9+, lrig1+, and blimp1+ epithelial cells and LRCs across the ocular surface epithelium. Using the 3-D objects counter in ImageJ, the co-localization of sox9, lrig1, and blimp1 with epithelial cells and LRCs was quantified in the (A) limbus (n = 1987 average nuclei), (B) cornea (n = 3407 average nuclei), and (C) fornix conjunctiva epithelium (n = 2386 average nuclei) of the H2B-GFP/K5tTA mouse by immunofluorescence tomography. The limbus and fornix conjunctiva exhibited lrig1+ LRCs and blimp1± or sox9± LRCs. No LRCs were observed in the central corneal epithelium.