Table 1.
Overview of D. immitis tissues analyzed in current study
| Sample | Total reads (millions) | High quality million mapped reads | High quality million mapped reads (combined) | Average coverage (FPKM)/transcript | % of all predicted D. immitis transcripts detected | Number of D. immitis proteins detected | ||
|---|---|---|---|---|---|---|---|---|
| FBW | 1.8 | 1.5 | 2.6 | 37.5 | 55 | 1800 |
|
2408 |
| 1912 | ||||||||
| 1.3 | 1.1 | |||||||
| 1324 | ||||||||
| FU | 1.9 | 1.6 | 3.8 | 66.8 | 74 | 3341 | ||
| 2.5 | 2.2 | |||||||
| FH | 1.5 | 1.3 | 2.6 | 25.4 | 56 | 1100 | ||
| 1.5 | 1.3 | |||||||
| FI | 1.8 | 1.5 | 3.3 | 38.2 | 60 | 202 | ||
| 2.1 | 1.8 | |||||||
| MBW | 7.4 | 5.1 | 8.5 | 70.1 | 64 | - | ||
| 5.1 | 3.4 | |||||||
| MI | 7.6 | 4.5 | 4.5 | 58.7 | 69 | - | ||
| MT | 7.4 | 4.7 | 4.7 | 61.8 | 76 | - | ||
| MS | - | - | - | - | - | 35 | ||
| MH | - | - | - | - | - | 1183 | ||
D. immitis tissues (FBW female body wall, FU female uterus, FH female head, FIfemale intestine, MBW male body wall, MI male intestine, MT male testis, MS male spicule, MH male head) analyzed by RNA-seq and/or MS-based proteomics. Values from each biological replicate (tissue samples from different worms) analyzed by each method are presented. Technical replicates were pooled prior to further analysis. Total number of unique proteins identified are presented for each tissue (bracketed for FBW biological replicates)