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. 2015 Nov 11;15:896. doi: 10.1186/s12885-015-1907-4

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© Werner et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Analysis of IRX2 mRNA and protein expression in breast cancer cell lines. a Quantitative gene expression analysis of IRX2 in different breast cancer cell lines. The data shown are the average fold change normalized to RPLP0 and UHR expression of three independent experiments; the error bars represent the standard deviation of the mean. b IRX2 protein expression in the same panel of human breast carcinoma cell lines was determined by Western blot analysis using a polyclonal RAI2-specific antibody that recognizes an internal IRX2 epitope. Equal loading was demonstrated using an antibody recognizing HSC70