Fig. 2.

Analysis of IRX2 expression and chemokine secretion in breast cancer cell lines over expressing IRX2. a Recombinant IRX2 protein expression in BT-549 and Hs578T cells that were transduced with retro-virus containing HA-tagged IRX2-cDNA. IRX2 protein expression was determined by Western blot analysis using a HA-specific antibody. Equal loading was demonstrated using an antibody recognizing HSC70. b Results from Proteome Profiler Antibody Array for determination of relative chemokine secretion. Cell culture supernatants from BT-549 and Hs578t cells over expressing the IRX2 protein were analyzed and compared with the vector control supernatants. c Normalized pixel intensities from Proteome Profiler Antibody Arrays. Individual signal intensities were measured using ImageJ software and normalized to the mean signal intensities of all reference spots