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. 2015 Nov 11;15:896. doi: 10.1186/s12885-015-1907-4

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© Werner et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of cell migration and proliferation. a Migration analysis of BT-549 cells over expressing IRX2 and from control cells by wound healing assay. Closure of wounded cell layer was examined by time-lapse videomicroscopy and evaluated using the Volocity 6 software. b Analysis of cell proliferation of BT-549 cells over expressing IRX2 and control cell lines by MTT assay. Five thousand cells were plated in quadruplicates and incubated under normal culture conditions for the indicated time span before measurement. Error bars represent the standard deviation of the mean of three independent experiments