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. 2015 Nov 11;15:896. doi: 10.1186/s12885-015-1907-4

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© Werner et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of cell migration. a Migration analysis of parental BT-549 and MDA-MB-231 cells using Boyden chamber assay. Cell culture supernatants from BT-549 cells over expressing IRX2 or from control cell lines were used as chemoattractant for 24 h. Error bars represent the standard deviation of the mean of three independent experiments done in triplicates. P-values were calculated by the two-sided Student’s t-test (*, p < 0.05). b Migration analysis of BT-549 cells over expressing IRX2 and from control cells using Boyden chamber assay Standard cell culture medium was used as chemoattractant for 24 h. Error bars represent the standard deviation of the mean of three independent experiments done in triplicates. P-values were calculated by the two-sided Student’s t-test (*, p < 0.05)