(A) Northern blot. sNrp1 is detected with a, b, and intron probes in the liver (L); while Nrp1 is detected with a, b, and 3'UTR probes in kidney (K), liver, and heart (H). (B) Western blot of mouse organs. GAPDH = loading control. Skin (S), Brain (B). (C) Western blot. N-terminal anti-Nrp1 detects Nrp1 in adult liver and heart and sNrp1 in liver. C-terminal anti-Nrp1 detects Nrp1 in heart and liver. (D) Northern blot. Nrp1 mRNA in adult and neonatal (P3) heart and liver. sNrp1 in the adult liver. (E)
sNrp1 is upregulated in P20 livers and remains elevated. (F)
sNrp1 is slightly upregulated in P17-20 kidneys. Nrp1 is more abundant than sNrp1 in kidney. Actin = loading control. (G) VEGF (blue nuclei) in P17 VEGFLacZ mouse liver. Eosin counterstain. Scale bar = 0.1 mm.