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. Author manuscript; available in PMC: 2015 Nov 12.
Published in final edited form as: Pathology. 2014 Aug;46(5):416–423. doi: 10.1097/PAT.0000000000000121

Figure 3. NRP1 binds HGF.

Figure 3

The weight of the overall mouse (A), heart (B), liver (C), and kidney (D) were measured at various time points after birth. All weights plateaued around 20 days. (E) Graph of liver weight following PHx. (F) VEGF (blue nuclei) is expressed in many hepatocytes 4 days following PHx. Eosin counterstain. Scale bar = 0.1 mm. (G-K) HGF binding assay using biotinylated human HGF and avidin-FITC. HGF binds MDAMB231 cells (G) and PAE NRP1 cells (H, J), but not PAE cells (I). sNRP1 competes the binding of HGF to NRP1 (K). Note green color is on the cell membrane.