Time-course of effect of lenalidomide on generation of cytokines by T cells of young subjects. Conditions, anti-TCR antibody stimulation, methods of quantification of cytokines, and calculation of statistical significance were the same as in Figure 1. Each column and bar depicts the mean ± S.E.M. of the results of cytokine ELISAs of supernates from T cells of six healthy young subjects (three women and three men). The mean ± S.D. of control (100%) values for IFN-γ, IL-2 and IL-17, respectively, were 3901 ± 1087, 535 ± 85 and 296 ± 92 pg/ml on day 1,33,623 ± 8934, 29 ± 3.4 and 1149 ± 372 pg/ml on day 3, and 49,095 ± 13,501,18 ± 1.8 and 1521 ± 376 pg/ml on day5.A=IFN-γ, B = IL-2 and C = IL-17.