Time-course of effect of lenalidomide on generation of cytokines by T cells of old subjects. Conditions, anti-TCR antibody stimulation, methods of quantification of cytokines, and calculation of statistical significance were the same as in Figure 1. Each column and bar depicts the mean ± S.E.M. of the results of cytokine ELISAs of supernates from T cells of six healthy old subjects (three women and three men). The mean ± S.D. of control (100%) values for IFN-γ, IL-2 and IL-17, respectively, were 1786 ± 1065, 371 ± 247 and 79 ± 57 pg/ml on day 1,26,804 ± 15,216,15 ± 10 and 354 ± 233 pg/ml on day 3, and 115,839 ± 52,211,13 ± 8 and 721 ± 454 pg/ml on day 5.A=IFN-γ, B = IL-2 and C=IL-17.