Table 3.

Lenalidomide effects on LPS-stimulated macrophage generation of TNF-α.

Concentration of lenalidomide (nM) (percentage of control, mean±S.D.)
	10	30	100	300	1000
Incubation time (h)
6	91 ±14	78±13	67±7.8*	65±4.5**	46 ±14*
24	69±11*	54±17*	35 ±4.0**	33±1.5**	20±4.5**
48	73 ±12	57±2.5**	48±3.2**	36±4.6**	36 ±7.6**

Each value is the mean +S.D. of the results from studies of two adults of ages 21–45 and two adults of ages N65 years. Production of TNF-α evoked by 100 ng/ml of LPS in the absence of lenalidomide (100% control) ranged from 4439 to 6039 pg/ml, 1843 to 2038 pg/ml, and 222 to 308 pg/ml after 6, 24 and 48 h of incubation. The significance of decreases in TNF-α generation induced by lenalidomide relative to the control (100%) value were calculated by a two-sample t test;

⁺=pb0.05,

^*=pb0.01 and

^**=pb0.001.