Fig. 1.

Characteristics of porcine L-MSCs. a Morphology of porcine L-MSCs. Porcine L-MSCs exhibit characteristic fibroblast-like morphology. b Colony forming unit-fibroblast assay. L-MSCs were cultured at 100 cells/well in a six-well plate. Single cells proliferated and formed colonies as shown by Giemsa staining. c In vitro proliferation potential of L-MSCs. L-MSCs were suspended in DMEM containing 10 % FBS and cultured in a 96-well plate. At indicated intervals, cell proliferation was measured by MTT assay. Optical density (OD) values at each time point are mean ± SD of L-MSCs isolated from three different pigs and cultured in triplicate wells