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Splicing rules predict efficient splicing reactions in cells. (A) Ion traces and estimated amounts of the test peptide SGYSGIFSVEGK (10 fmol on column), the spliced peptides [YLGN][RLPSV] and [YLGD][RLPSV], and the HLA-A*0201 binding peptide YLWGRPLSV, as eluted from the cell surface of JY or MelJuso-A2 cells. (B) MS/MS spectrum of peptide [YLGD][RLPSV] as eluted from the cell surface of JY cells.
