Table I. MS peak areas of ligation products and the corresponding hydrolysis products identified in digestion mixtures of peptide N1.1.
| Incubation Time (nmol Peptide/10 μg Proteasome) | MS Peak Area/10 μg Proteasome |
IC50 | |||
|---|---|---|---|---|---|
| 20 h |
8 h |
||||
| 100 | 200 | 400 | 150 | ||
| GSSF | 1,644 | 1,972 | 2,722 | 1,960 | |
| GSSF-GLASYKIFKIEKGKV | 176 | 398 | 578 | 400 | ND |
| GSSF-GLASYKIFKI | 88 | 114 | 136 | 104 | ND |
| GSSF-GLASY | 334 | 416 | 434 | 477 | >1 mM |
| GSSF-KIFKIEKGKV | 126 | 152 | 236 | 193 | ND |
| GSSF-KIFKIEKG | 82 | 86 | 94 | 79 | ND |
| GSSF-KIFKI | 776 | 888 | 1,106 | 1,304 | 39 μM |
| GSSFKIF | 134 | 158 | 270 | 319 | ND |
| GSSFTI | 3,630 | 3,764 | 3,962 | 2,923 | |
| GSSFTI-GLASYKIFKI | 18 | 40 | 64 | 38 | ND |
| GSSFTI-GLA | 44 | 50 | 108 | 112 | ND |
| GSSFTI-KIFKIEKGKV | 58 | 80 | 116 | 102 | ND |
| GSSFTI-KIFKI | 186 | 242 | 452 | 334 | ND |
| GSSFTI-KIF | 236 | 256 | 344 | 333 | ND |
| GSSFTIMTD | 5,446 | 5,898 | 5,892 | 4,572 | |
| GSSFTIMTD-GLASYKIFKIEKGKV | 42 | 56 | 94 | 124 | ND |
| GSSFTIMTD-GLASYKIFKI | 0 | 14 | 18 | 23 | ND |
| GSSFTIMTD-GLASY | 0 | 0 | 0 | 40 | 49 μM |
| GSSFTIMTD-KIFKI | 30 | 56 | 106 | 109 | ND |
| TIMTD-KIFKI | 28 | 42 | 0 | 64 | 6.8 μM |
| GSSFTIMTDGPSD | 13,732 | 16,356 | 17,014 | 12,156 | |
| GSSFTIMTDGPSD-KIFKIEKGKV | 54 | 118 | 136 | 164 | ND |
| GSSFTIMTDGPSD-KIFKI | 74 | 202 | 292 | 328 | ND |
| GPSD-KIFKI | 70 | 98 | 122 | 183 | ND |