FIGURE 1:

Ni-NTA-nanogold labeling of recombinant Chlamydomonas OAD complex. (A) Diagram of the LC1-C-His construct and negatively stained EM images of the gold-labeled LC1-C-His OAD complex. Orange arrowheads indicate gold particles. Gold-labeled stalk tips sometimes had extra densities (indicated by red arrowheads). Bar, 50 nm. (B) Schematic diagram of the LC2-C-His construct and negatively stained EM images of the gold-labeled LC2-C-His OAD complex. Orange arrowheads show gold particles. Bar, 50 nm. (C) Fractions of the Ni-NTA-nanogold binding. Negatively stained OAD molecules were classified as tail binding, middle binding, head binding, or no binding. Molecules gold-labeled at a position between the tail and the head were classified as middle binding.