Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 15;26(23):4236–4247. doi: 10.1091/mbc.E15-05-0289

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ichikawa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 6: — Binding of γ stalk and LC1 to the microtubule. (A) The molar ratios of γ stalk and tubulin dimer in the precipitated fractions (ppt) are plotted against the free γ stalk concentrations in the supernatant fraction (sup) for γ stalk (red) and γ stalk-LC1 complex (green). The molar ratios of LC1 and tubulin dimer in the precipitated fraction and the free LC1 concentrations in the supernatant fraction are plotted for LC1 (blue). See also Supplemental Figure S8. When X < K_d, Y = B_maxX/(K_d + X) can be approximated by Y = B_maxX/K_d. Thus the slopes (B_max/K_d) were determined from the linear curve fitting. (B) Comparison of affinities (B_max/K_d) for microtubules. Error bars represent the SE in the fitting. The values of B_max/K_d are also summarized in Table 1. Statistical analyses were performed using one-way ANOVA, followed by Tukey's multiple comparisons test (*p < 0.0001).