Fig 6. Self-assembly of MinE^1-31 on SLBs.

(A–C) Assembly of MinE^1-31 on a high-coverage area of the SLBs. The peptide concentration was 24 μM. (E–G) Assembly of MinE^1-31 on broken SLBs. The time for each set of data is indicated at the upper-right hand corner of the images. Notice that (G) was taken before (E) and (F). Newly assembled fibrils can be observed between (A) and (B) and between (E) and (F). The peptide concentration used was 12 μM. Graphs (B) and (C) and (F) and (G) are images of (A) and (B) at higher resolution, respectively. Graphs (D) and (H) show the height profiles corresponding to the green lines in images (C) and (G). Experiments were performed in imaging buffer A. Red double arrows indicate the separation distance between protofibrils. Green double arrow indicates the separation distance between fibrils. Purple arrows indicate the newly formed fibrils comparing to the previous images. Blue line indicates the thickness of supported lipid bilayer.