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. 2015 Nov 12;10(11):e0142875. doi: 10.1371/journal.pone.0142875

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© 2015 Sum et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — DNA ladder (lane 1); pNN9 (CCC) (lane 2); DNase I exposed pNN9 (CCC) (lanes 3 & 4); CCC/16-3-16 lipoplexes at 2:1, 5:1, and 10:1 charge ratios (lanes 5–7); CCC/16-3-16/DOPE lipoplexes at 2:1, 5:1, and 10:1 charge ratios (lanes 8–10); 16-3-16/DOPE (lane 11); DNA ministring (LCC) (lane 12); DNase I exposed DNA ministring (LCC) (lanes 13 & 14); LCC/16-3-16 lipoplexes at 2:1, 5:1, and 10:1 charge ratios (lanes 15–17); LCC/16-3-16/DOPE lipoplexes at 2:1, 5:1, and 10:1 charge ratios (lanes 18–20). Equal loading of the recovered DNA solution after DNase I exposure indicated improved DNA recovery for ministring (LCC) derived lipoplexes. DNA/16-3-16 lipoplexes conferred better DNA recovery after DNase I degradation over DNA/16-3-16/DOPE lipoplexes across all of the tested charge ratios.