Table 1. Forward (F) and reverse (R) primer sequences of canine genes included in the present study and used for polymerase chain reaction with the corresponding annealing temperature and product length.

GENE AND PRIMER SEQUENCES (5´-3´)	EXON	TEMP. ANNEALING	PRODUCT LENGHT
KRAS
F: CTCATCTGTGGTCAACTGAA	1	60°C	466 bp
R: AGCCAATGGAACCCAAGTA
IDH1
F: TGGCACTGTCTTCAGGGAAGCTAT	2	70°C	163 bp
R: TGGGCAACCAAGGACAGGAAAA
IDH2
F: CTCCATCTCTGTCCTCGTAGAGT	4	67°C	343 bp
R: TTAGCACCGCTGCCATCCTTT
NRAS
F: TCTCTAGTTGTGGCTCGCCCATTA	1	65°C	223 bp
R:CAAAAGCCAGAGGTAGGGTCAGT
F:GCTAGGAGCTTATCTAACCTTGGC	2	60°C	367 bp
R: TGCGGTATCCTCATTTCCTGTTCC
SF3B1
F: ACTGGAGGATCAAGAGCGTCAT	13	67°C	1101 bp
R: GCTGTCGTGTTACGGACATACT
F: ATGCTAGAGTGGAAGGTCGAGA	14	67°C	855 bp
R: TGTGTTGGCGGATACCCTT
F: GACCATTAGCGCTTTGGCCATT	15–16	67°C	529 bp
R: GTTCCACAACACTGCTTCACCA
TET2
F: AGCCTGATGGAACAGGATAGA	3	60°C	782 bp
R: GCCTGACTGTTAATGGCA
F:CAAGAAAGTAATCCAGGCAAAGGC	3	60°C	718 bp
R: AATACCGTTCAGAGCTGCCA
F: CCTGTCCCTTCCAGAAACCAGAAA	3	60°C	605 bp
R: TGTTGGGTTATGCTTGAGGTGTTC
F: CCCCAACCAAAGTAACACAGCTCT	3	60°C	702 bp
R: GCTTTGGATGAAGGGTCTGTCTTG
F: GGCATCACTGCGGTCAGTTCTT	3	60°C	715 bp
R: ATTCTGTCCTTGCTCCAATCCCA
F: TCCCAAGGCAACAATGATCAGC	3	60°C	760 bp
R: GGGGTGGAATCTCTTGCTTAGTTG
F: CTCCCCAGAAGGACATTCAAAAG	3	60°C	784 bp
R: CTCTCTTGCACAGCACAAGCAT
F: GGATAAGCTTTGTGGATGTAGCCT	4	60°C	371 bp
R: GCTCGCAGACTATTAGTCCTGT
F: TCCAGTTTGCTTGGCTTAGAC	5	60°C	380 bp
R: GAGCAACGTTCATTTCAACTAGC
F: AATGCCCTAGTTGTGACCCAG	6	60°C	421 bp
R: AAATGTCGGTTCAACTCCCTTCCC
F: CCAGAATCCAAGATTGGTAGCC	7	60°C	295 bp
R: GACTGCTTACTTCATCTGTACTCA
F: TCATTTGGATCTAGGCTGTAGGGG	8	65°C	336 bp
R: AACAGAACACTGTGGCTTCACT
F: CGAGAGTCTTTCTGACCTGTTC	9	60°C	398 bp
R: AAGGTCACCTTTGCAACAGC
F: AGGCATGTCACTAATCTGGTCCAA	10	60°C	638 bp
R:GGGACTTCAGGGAAGATTCTGGTA
F: GGGGTTCTCACATACATTTAAGCA	11	65°C	920 bp
R: GAGCTGTTGAACATGCCTGG
F: ACTTCATGGGAGCCACCTCTAGAT	11	60°C	853 bp
R: AGACAGGTTGGTTGGTTGGTTGTG