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. 2015 Nov 12;10(11):e0143022. doi: 10.1371/journal.pone.0143022

Fig 1. Characterization of GhWRKY41 as a transcriptional regulator.

Fig 1

(A) Schematic diagrams of the p35S::GhWRKY41-GFP fusion construct and the control p35S::GFP construct. (B) Transient expression of the p35S::GhWRKY41-GFP and p35S::GFP constructs in N. benthamiana. Green fluorescence was observed using a confocal microscope five days after transformation. (C) GhWRKY41 demonstrates transactivation activity. The full-length ORF of GhWRKY41 was subcloned into pGBKT7, and transformed yeast was selected on both SD-Trp and SD-Trp-His-Ade media. Positive transformants were further identified by spotting serial yeast dilutions (1/1, 1/10 and 1/100). The triangle indicates the dilutions from 1 to 100. (D) Sequences of three tandem W-boxes (TGAC) and mW-boxes. (E) Yeast one-hybrid assays. (1) pAbAi-Wbox + pGADT7. (2) pAbAi-Wbox + pGAD-GhWRKY41. (3) pAbAi-mWbox + pGADT7. (4) pAbAi-mWbox + pGAD-GhWRKY41.