Table 3. The relative expression of prtA, psaB, psaC, psaA, and pcpA genes was normalized with the housekeeping gene gyrA.

The log² fold increase is relative to the expression in the D39 wild-type grown in CDMchelex with 0.3 mM Ni²⁺ to that with 0 mM Ni²⁺. Standard deviation of three independent replications is given in parentheses.

Gene tag a	Function b	Fold Raito
spd_0558	Cell wall-associated serine protease PrtA	4.53 (1.22)
spd_1461	Manganese ABC transporter, ATP-binding protein, PsaB	2.83 (0.24)
spd_1462	Manganese ABC transporter, permease protein, PsaC	3.15 (0.30)
spd_1463	Manganese ABC transporter, ATP-binding protein, PsaA	5.88 (1.55)
spd_1965	Choline binding protein PcpA	10.53 (3.09)

^aGene numbers refer to D39 locus tags.

^bD39 annotation/TIGR4 annotation. [34,62]