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. 2015 Nov 1;11(11):1337–1347. doi: 10.7150/ijbs.12996

Figure 1.

Figure 1

YZ-LY-0 inhibited EV71 proliferation. (A) The chemical formula of YZ-LY-0.(B) The inhibitory effect of YZ-LY-0 on EV71 RNA measured by qRT-PCR. RD cells were infected by EV71 virus at a MOI of 1, with or without treatment at various concentrations of YZ-LY-0 (0.039-10 μM) for 24 h. The levels of EV71 RNA were quantified by qRT-PCR and the data was expressed as the percentage of the level of EV71 RNA compared with viral RNA in the cells without YZ-LY-0 treatment. Each data point represents the average of three replicates. (C) Concentration-dependent suppression of EV71 proliferation following treatment with YZ-LY-0. RD cells were infected by EV71-GFP virus at a MOI of 1, with or without treatment by various concentrations of YZ-LY-0 for 24 h. Results for concentrations 0.16μM, 0.63μM, 2.5 μM and 10μM are shown. DAPI was used to visualize the nuclei (top panel), mark up the host cells, and EGFP was used to monitor the virus growth (bottom panel). (D) Dose-dependent reduction of EV71 VP1 expression. The concentration of YZ-LY-0 in lanes 1-7 was 5.00, 2.50,1.25,0.63, 0.31 0.16,and 0 μM, respectively. The expression level of GAPDH was not affected by the treatment with YZ-LY-0.