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. 2015 Nov 1;11(11):1337–1347. doi: 10.7150/ijbs.12996

Figure 2.

Figure 2

The anti-EV71 activities of YZ-LY-0 at different addition times. (A), (B) and (C) Inhibition of EV71 (FY)-Luc pseudotype virus infection of RD cells by YZ-LY-0 (2 μM), NITD008 (2 μM) and GPP3 (1 pM) at various addition times. EV71 pseudotype virus was used to infect RD cells in a 96-well plate and YZ-LY-0 was added simultaneously (0 hpi) or before and after viral infection as indicated on the figure (-6, -4, -2, 0, 2, 4, 6, 8 and 10 h p i). The luciferase levels were quantified by measuring the firefly luciferase activity in relative luminescence units (RLU) at 24 h p.i. (D) The EV71 subgenomic replicon RNA lacking the P1 region was transfected into RD cells. YZ-LY-0 (2 μM), NITD008 (2 μM) and GPP3 (1 pM) were added 4h prior to transfection. The luciferase levels were quantified by measuring the firefly luciferase activity in relative luminescence units at 24 h p.i. All results were presented as the average results of three independent experiments and SDs (n = 3) are presented.