Figure 4.

Quantification of the anti-EV71 effect of YZ-LY-0 in different cell lines. (A), (D) and (G) RD, 293T and Vero cells were treated with YZ-LY-0 and infected with EV71 strain Fuyang virus. EV71 RNAs were measured using qRT-PCR at 24 hpi. (B), (E) and (H) RD, 293T and Vero cells were treated with YZ-LY-0 and infected with the living virus of EV71 strain Fuyang. The virus titers in the supernatants were determined at 72 hpi. All results are presented as the average results of three independent experiments and SDs (n = 3) are presented. (C), (F), and (I) Cytotoxicity in different cell lines. RD, 293T and Vero cells were incubated with YZ-LY-0 at the indicated concentrations for 24 h. The cell viability was measured using a WST-1 based assay.