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. 2015 Oct 25;6(12):1295–1305. doi: 10.7150/jca.13176

Figure 5.

Figure 5

MiR-182 negatively regulates FOXO1 in LNCaP cells. (A), Western blot analysis of FOXO1 protein expression in LNCaP cells, stably transduced LNCaP cells with empty vector (Vector), and with miR-182-expressing vector (miR-182). FOXO1 levels inversely correlate with miR-182 expression. LNCaP-miR-182 cells were also transfected with antisense miR-182 or control inhibitors. Inhibition of miR-182 increases FOXO1 levels. Densitometric quantification of FOXO1 relative to β-Tubulin from three independent experiments is graphically depicted. (B), Western blot analysis of putative targets of miR-182, FOXO3 and MITF, in LNCaP, Vector, and miR-182 cells. (C), LNCaP-miR-182 cells transfected with FOXO1 siRNA shows that miR-182-mediated downregulation of FOXO1 was comparable to that achieved by FOXO1-specific siRNA. Densitometric quantification of FOXO1 relative to β-Tubulin from three independent experiments is graphically depicted.