Figure 1.

BRAF^V600E typing in melanoma. Five primary melanoma cell lines (MEL-2, MEL-V, KFM, GLM-2, and 3MM) and three established melanoma cell lines (SK-MEL-28, SK-MEL-37, SK-MEL-103) were typed for BRAFV600E status and allelic discrimination plot shown. Allele Y refers to the BRAF^V600E allele, Allele X refers to the wild type BRAF allele. Typing was performed in duplicate samples, lines denoted in blue represent cells containing only mutant alleles, green denotes cell containing both mutant and wild type alleles, and red denoted cells only containing wild type alleles.