Fig. 6.

A mutation in AT00_17125 leads to a translucent morphology resulting from a reduction in CPS biosynthesis. a In-frame deletion of AT00_17125 was confirmed by PCR using four sets of primers. Lanes 1, 3, 5 and 7 used DNA from the wild-type strain, and lanes 2, 4, 6, and 8 used DNA from the deletion mutant. Lanes 1 and 2 show DNA amplified using the primer pair 17125-SF/−SR, lanes 3 and 4 after using 17125-SF/−LR, lanes 5 and 6 using 17125-LF/−SR, and lanes 7 and 8 using 17125-LF/−LR (Table S1). The expected product sizes were 1491, 2993, 2871, and 4373 bp for the wild-type and 382, 1884, 1762, and 3264 bp for Δ08765. The expected product sizes were 1389, 3030, 3869, and 5510 bp for the wild-type strain and 559, 2200, 2254, and 4680 bp for Δ17125, respectively. b Morphology of the wild-type and Δ17125 strains characterized by transmission electron microscopy. c Δ17125 showed a translucent morphology in SW-LB medium. Complementation of wild-type AT00_17125 via plasmid pBBR1MCS-17125 (p-17125) restored the phenotypes of Δ17125 and of the translucent variant to opaque