Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Relevant characteristics | Source |
|---|---|---|
| E.coli strains | ||
| WM3064 | RP4(tra) in chromosome, DAP− | (Dehio and Meyer 1997 ) |
| Isogenic mutants of Pseudoalteromonas lipolytica SCSIO 04301 | ||
| Δ08765 | In-frame deletion of AT00_08765 | This study |
| Δ17125 | In-frame deletion of AT00_17125 | This study |
| Δ17170 | In-frame deletion of AT00_17170 | This study |
| Δ17220 | In-frame deletion of AT00_17220 | This study |
| Δ08765 ΔbcsZB | In-frame deletion of AT00_08765, bcsZ, bcsB | This study |
| Plasmid | ||
| pK18mobsacB-ery | pK18mobsacB containing the erythromycin-resistant gene from pHT304, Kanr, Eryr | (Wang et al. 2015) |
| pK18mobsacB-ery-08765 | Recombinant plasmid for deleting AT00_08765 | This study |
| pK18mobsacB-ery-17125 | Recombinant plasmid for deleting AT00_17125 | This study |
| pK18mobsacB-ery-17170 | Recombinant plasmid for deleting AT00_17170 | This study |
| pK18mobsacB-ery-17220 | Recombinant plasmid for deleting AT00_17220 | This study |
| pK18mobsacB-ery-bcsZB | Recombinant plasmid for deleting bcsZB | This study |
| pBBR1MCS-Cm | Broad-host-range vector containing the chloramphenicol-resistant gene from pWD2 | This study |
| pBBR1MCS-08765 | AT00_08765 cloned into pBBR1MCS-Cm | This study |
| pBBR1MCS-17125 | AT00_17125 cloned into pBBR1MCS-Cm | This study |
Erythromycin (25 μg/mL) and chloramphenicol (30 μg/mL) were used to maintain the pK18mobsacB-ery and pBBR1MCS-Cm plasmids, respectively