Figure 1.

Physical processes responsible for local recruitment of pmCRY2. (a) CRY2-mCherry TIRF images before illumination (top) and after six local activations in the indicated red box (middle) and after six activations in the indicated green box (bottom). (b) Quantification of the relative increase of signal in the red and green region over time. (c) Scheme of the biophysical processes involved in CRY2-mCherry localization at the plasma membrane. Inactive cytoplasmic CRY2 (solid red circles) changes conformation upon illumination into an active state (open red circle) that diffuses to the membrane and binds CIBN (solid green circles). (Yellow region) Evanescent TIRF field. (Black arrows) Diffusion-limited membrane recruitment, lateral diffusion, and dimer dissociation altogether representing the cycle of CRY2 in a steady and localized stimulation (blue cone of light). To see this figure in color, go online.