Figure 6.

Local activation of Cdc42 in fibroblast cell generates membrane activity and cell barycenter displacement. (a–c) DIC images (a and c) and TIRF images (b) of a fibroblast illuminated locally by a rectangular ROI (1 pulse every 20 s, blue region). (a) The cell is represented before the activation routine. Scale bar = 10 μm. (b) TIRF images before, at 1 min, and 50 min after activation. (c) Zoom-in of the black area of (a) for 8, 17, 30, and 50 min after the beginning of the activation routine. (Black arrows) Presence of filopodia; (green arrows) localization of vesicles. (d) Quantification of pmCRY2 recruitment in the activation area (green), of membrane activity (blue) and of cell barycenter displacement (red) over time normalized between 0 and 1. To see this figure in color, go online.