Skip to main content
. 2015 Nov 13;5:16449. doi: 10.1038/srep16449

Figure 4. Notch1 and Notch3 cooperate to regulate the vascular basement membrane.

Figure 4

(A–C) High magnification images taken at the capillary plexus of P5 retinas whole-mount immunostained with isolectin B4 (red) and collagen IV (green). (A’–C’) Corresponding collagen IV staining in grey. (D–F) P5 retinal capillary plexus stained with isolectin B4 (red) and laminin (green). (D’–F’) Corresponding laminin staining in grey. (G) Mmp14 transcript levels in P5 retinas measured by quantitative real time PCR, normalized to gapdh and relative to control. (H) Western blot for MMP14 and vinculin (loading control) on HBVP protein lysates. (I) MMP14 protein levels in HBVP cell lysates, normalized to vinculin and relative to control. n≥3; *P < 0.05, **P < 0.0001. Data are mean±s.e.m. Scale bars: 25 μm.