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. 2015 Nov 13;5:16595. doi: 10.1038/srep16595

Figure 3. FACS purification of mCherry+ cells for culture and analysis.

Figure 3

(a) FACS setup. Differentiated non-reporter H7 hESCs were used to set a threshold for mCherry fluorescence from A81-H7 cells. Density plots and histograms are displayed, with the percent of mCherry fluorescent cells indicated below. (b) Microscopy images of mCherry+ sorted cells one-day post FACS. Phase and fluorescence images are shown. Scale bar = 100 μm. (c) Fluorescence microscopy images of mCherry+ and mCherry- sorted cells. Cells were fixed and stained for BRN3B one day after sorting. Scale bar = 100 μm.