Figure 4.

Intersubunit hydrophobic cluster between P- and S6 helices in (a) closed and (b) open conformations. The side chains from one subunit are colored gray and those from an adjacent subunit are rendered as yellow sticks. Whole-cell recordings at +100 mV of TRPM8 currents induced with either cold (22°C), or icilin (10 μM), or menthol (500 μM) for HEK cells transfected with (c) Y⁹⁰⁵A, Y⁹⁰⁵F, or Y⁹⁰⁵W TRPM8 mutants, and (d) Y⁹⁰⁸A, Y⁹⁰⁸F, or Y⁹⁰⁸W TRPM8 mutants. Cells transfected with WT TRPM8 were used as control (ctrl). To see this figure in color, go online.