Figure 5.

Residues involved in conformation of P-helix and of SF-to-S6 EC loop. The closed conformation is colored red and the open state in cyan. The interactions between (a) E⁹⁰⁶ and R⁹⁵⁰ are rendered as sticks and (d) disulphide bond formed by C⁹²⁹-C⁹⁴⁰ are illustrated as transparent balls. Whole-cell recordings at +100 mV of TRPM8 currents induced with either cold (22°C), or icilin (10 μM), or menthol (500 μM) for HEK cells transfected with (b) E⁹⁰⁶A or E⁹⁰⁶Q TRPM8 mutant (c) R⁹⁵⁰E TRPM8 mutant (e) C⁹⁴⁰R TRPM8 mutant and (f) C⁹⁴⁰G TRPM8 mutant. Cells transfected with WT TRPM8 were used as control (ctrl). To see this figure in color, go online.