Figure 4.
Virion surface area distributions at different levels of cholesterol extraction. Histograms of calculated viral surface area are plotted for unmodified, cholesterol-depleted, and cholesterol-replenished influenza virus. Median surface areas and 90% confidence intervals are 2.2 × 105 Å2 (1.1 × 105 to 4.4 × 105) for unmodified virus, 2.1 × 105 Å2 (1.5 × 105 to 4.1 × 105) for cholesterol-depleted virus, and 2.3 × 105 Å2 (1.2 × 105 to 5.0 × 105) for unmodified virus. Median surface areas and surface area distributions do not significantly differ among the three distributions (Wilcoxon Rank Sum test and Kolmogorov-Smirnov test with Bonferroni correction), although a statistically insignificant difference in the width of the main peak can be observed. To see this figure in color, go online.