Figure 1.

Cytochrome c structure and peroxidase activity. (a) X-ray structure of oxidized cyt-c (44). Labels mark the catalytic sites of peroxidase-active protein (heme and Tyr-67). Cyt-c’s six alanines are also indicated. (b) Generation of the fluorescent oxidation product resorufin by 0.5 μM cyt-c. Measurements were performed at room temperature in the absence (dashed line) and presence of DOPC LUVs (gray solid line) or DOPC/TOCL (4:1) LUVs (black solid line). (c) Relative peroxidation rates obtained from linear fits to the data in (b). To see this figure in color, go online.