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. 2015 Nov 4;109(9):1873–1884. doi: 10.1016/j.bpj.2015.09.016

Figure 2.

Figure 2

1D MAS NMR spectra of oxidized and reduced U-13C,15N cyt-c bound to TOCL/DOPC LUVs. (a and b) 1H-13C CP and direct excitation 13C spectra of oxidized and reduced membrane-bound cyt-c, at 271 K. The spectra in each panel are plotted with absolute intensities, and were processed with 25 Hz exponential line broadening and fourfold zero-filling. (c) 1H-13C CP spectrum at 257 K, showing mostly lipid signals. (d) CP spectrum at 250 K, and (e) 236 K, with much increased protein signals (up arrows). (f) 1D 1H-13C refocused INEPT spectrum at 257 K, with mostly lipid peaks (headgroups marked). (g and h) INEPT data at 250 K and 236 K, showing loss of lipid headgroup and acyl chain signals, respectively (down arrows). (i) 1D 1H spectrum at 257 K, with intense liquid water peak. (j) 1D 1H spectrum at 250 K, after freezing of solvent (down arrow). (k) 1H 1D at 236 K, showing a loss of acyl chain signals (down arrow). Data obtained at 600 MHz (1H) and 8.33 kHz MAS, and processed with a cosine-bell apodization function and zero-filling to twice the original size. Selected spectra are scaled as indicated. To see this figure in color, go online.