Figure 3.

USP4 Regulates CtIP Recruitment to DNA Damage Sites

(A) NBS1 recruitment to laser-line micro-irradiation induced DNA lesions in Cyclin A-positive U2OS cells that were treated with USP4-targeting siRNAs was not reduced compared to the siRNA control. Nuclei were outlined, and the scale bar indicated 10 μm.

(B) CtIP recruitment to laser-line micro-irradiation induced DNA lesions in Cyclin A-positive U2OS cells was reduced after USP4 siRNA treatments. RAD50 siRNA treatment (see Figure S3A for RAD50 depletion) was the positive control (mean ± SEM; n = 3; ^∗∗p < 0.01). Nuclei were outlined, and the scale bar indicated 10 μm. See also Figure S3.