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. 2015 Nov 5;60(3):362–373. doi: 10.1016/j.molcel.2015.09.019

Figure 4.

Figure 4

USP4 Interacts with CtIP and MRN via Its C-Terminal Insert Region

(A) Endogenous USP4 immunoprecipitation (IP) from 293FT cell extracts retrieved RAD50 and MRE11.

(B) Full-length (FL) GFP-FLAG-USP4 IP from U2OS cell lysates retrieved CtIP, RAD50, and MRE11.

(C and D) (C) GFP-FLAG-MRE11 or (D) GFP-CtIP immunoprecipitations from 293FT cell extracts retrieved USP4.

(E) Schematic view of full-length (FL) USP4 with indicated structural domains. USP4 deletion mutants and their ability to retrieve CtIP or MRN were indicated. Positions of cysteine, histidine and aspartic-acid that form the USP4 catalytic triad; the zinc-binding motif cysteine residues (CysXXCys); and the “thumb,” “fingers,” and “palm” catalytic subdomains were indicated.

(F) GFP-FLAG-USP4-F+I immunoprecipitations retrieved CtIP, RAD50, and MRE11 (See Figure S4C for corresponding inputs; all samples were run on the same SDS-poly acrylamide gel).

(G) GFP-FLAG-USP4-I immunoprecipitations retrieved CtIP, RAD50, and MRE11 (See Figure S4D for inputs). See also Figure S4.