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. 2015 Nov 5;60(3):362–373. doi: 10.1016/j.molcel.2015.09.019

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Auto-Regulated Ubiquitylation of Other USP-Family DUBs

(A) HA-ubiquitin (HA-Ub) immunoprecipitations (in presence of βME) retrieved USP4 CD, USP15 CD, and USP11 CD (wild-type USP4, USP15, and USP11 were not or weakly detected under these conditions).

(B) HA-Ub immunoprecipitations from U2OS cell extracts that were processed without βME retrieved modified forms of USP4 CD, USP15 CD, and USP11 CD (exp., exposure).

(C) HA-Ub immunoprecipitations from U2OS cell extracts retrieved USP15 CD-C451A less efficiently than USP15 CD.

(D) USP15 WT, WT-C451A, and CD-C451A but not CD immunoprecipitations from U2OS cell extracts (see Figure S7B for inputs) efficiently retrieved SMAD2/3. See also Figure S7.