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. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Mol Biosyst. 2015 Nov 10;11(12):3222–3230. doi: 10.1039/c5mb00423c

Figure 6.

Figure 6

Western blot analysis of FMRP interaction with G quadruplex within NR2B mRNA. The 5′-biotin labelled NR2B mRNA, PSD-95 mRNA and HCV RNA probes were incubated with E17 mouse brain lysate. The probes were precipitated with NeutrAvidin agarose beads and co-purified FMRP and SMN proteins were assessed by immunoblotting. Lane 1: 15% of brain lysate input showing existence of FMRP; Lane 2: No detection of FMRP or SMN when the probe is not present; Lane 3: No detection of FMRP and SMN using HCV probe; Lane 4: FMRP detected with PSD-95 mRNA probe. Lane 5: FMRP detected with NR2B mRNA probe.