Figure 1. Simplification of the WASP/Myosin complex.

(A) A diagram of the components of the WASP/Myosin complex (circled with dotted line), the coat protein Sla1 (green) that is hypothesized to recruit Las17, and proteins of the Actin Module (red) that are directly or indirectly recruited by WASP/Myosin complex NPF activity. Black arrows indicate known or theorized physical interactions between complex components while gray lines indicate regulatory relationships.

(B) Single frames from movies of wild-type (WT) cells and cells with various WASP/Myosin complex components deleted, all expressing Sla1-GFP and Abp1-RFP. A kymograph of a representative endocytic patch from each cell type is shown to the right of each image.

(C) Proportion of Sla1-GFP patches that internalize more than 3 pixels (194 nm). At least 10 patches from the mother cell (or two similar cells if fewer than 10 patches could be counted for a cell) were scored for movement and an average was obtained for each cell. At least fifteen cells on three or more separate days of imaging were analyzed. Values represent the mean +/− SEM. *P<0.001 vs MYO5 (myo3Δ).

(D) Growth of WASP/Myosin complex components on YPD plates. Starter cultures were diluted to an O.D₆₀₀ of 0.02 and plated. Two additional 1:5 dilutions were also plated. Plates were grown at the indicated temperature for 48 hours and imaged. See also Figure S1, Movie S1, Tables S1 and S2.