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. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Dev Cell. 2015 Nov 9;35(3):281–294. doi: 10.1016/j.devcel.2015.10.007

Figure 2. Endocytic roles of the Myo5 TH2 and SH3 domains.

Figure 2

(A) Diagram of the domains in Myo5 and each Myo5 variant analyzed in Figure 2.

(B) Maximum intensity projection of Myo5-GFP and Myo5-Vrp1-GFP patches (Green) imaged over the course of a 120 second movie in the medial focal plane. Each culture also contains cells with a reference Myo5-GFP and Mtw1-mCherry for identification. A maximum intensity Z-projection of Mtw1-mCherry fluorescence is shown to identify the reference cells in each field (red dots). Scale bar is 5 μm.

(C) Kymographs of representative endocytic patches from cells expressing Sla1-GFP and Abp1-RFP and the indicated Myo5 variant.

(D) Growth of Myo5 variants on YPD plates, as described for Figure 1D.

(E) Proportion of Sla1-GFP patches that internalize, as described in Figure 1C. *p<0.001 vs MYO5(myo3Δ); ** p<0.05 between bracketed variants; *** p<0.001 between bracketed variants.

(F) Histogram of normalized fluorescence intensity peaks of the indicated GFP-tagged Myo5 variants. Values represent the mean +/− SEM. * p<0.01 vs Myo5-GFP; **p<0.05 between bracketed variants; ***p<0.001 between bracketed variants. See also Figure S2, Movie S2, Tables S1 and S2.