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. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Dev Cell. 2015 Nov 9;35(3):281–294. doi: 10.1016/j.devcel.2015.10.007

Figure 3. Endocytic roles of the Myo5 motor domain.

Figure 3

(A) Diagram of each Myo5 variant analyzed in Figure 3.

(B) Kymographs of representative endocytic patches from cells expressing Sla1-GFP and Abp1-RFP.

(C) Proportion of Sla1-GFP patches that internalize, as described in Figure 1C. *P<0.001 vs MYO5 (myo3Δ).

(D and E) Growth of Myo5 variants on YPD plates, as described for Figure 1D.

(F) Proportion of Sla1-GFP patches that internalize, as described in Figure 1C. *P<0.001 vs MYO5 (myo3Δ).

(G) Growth of Myo5 variants on YPD plates, as described for Figure 1D.

(H) Proportion of Sla1-mCherry patches that internalize, as described in Figure 1C. *P<0.001 vs MYO5-GFP (MYO3). Myo5-GFP fluorescence was not imaged.

(I) Single frame from movies of sla2Δ cells expressing Sla1-GFP and Abp1-RFP with the indicated motor domain variants indicated. Also, kymographs from each cell are shown to the right of each image to show the extent of Abp1-RFP internalization relative to the Myo5-GFP signal. Image scale bar represents 5 μm. Vertical scale bar in kymograph is 1.0 μm, horizontal scale bar in kymograph is 20 seconds. See also Figure S3, Movies S3, Tables S1 and S2.