Figure 3. Multiple analysis commands can be combined.

(A) The Threshold Segmentation command input parameters are set to select regions of high intensity on the green channel. (B) The regions of interest (ROIs) selected by the command will be stored in separate files, with the file paths recorded in the results table. In addition, the image file names will be copied into the results table. (C) The ROI manager displays the resulting ROIs drawn on the original images. (D) The Region Statistics command is run on the results table from the Threshold Segmentation command. (E) This command then calculates the average values on each channel of the ROIs automatically selected by Threshold Segmentation. Since there were many ROIs selected, the results table contains more than one row for each image.